Longitudinal fluctuations of common antimicrobial resistance genes in the gut microbiomes of healthy Dutch individuals

• DOI: 10.1016/j.ijantimicag.2023.106716
• Publication Date: 2023-01-11T16:43:20Z
• Authors: Jakob J. Malin; Christian J.H. vo...; John Penders; Paul H.M. Savelkoul; Petra F.G. Wolffs
• Keywords: Resistome

The human gut microbiome is an important reservoir of antimicrobial resistance genes (ARGs), collectively termed the 'resistome'. To date, few studies have examined dynamics resistome in healthy individuals. Previously, authors observed high rates ARG acquisition and significant abundance shifts during international travel. In order to provide insight into commonly occurring dynamics, this study investigated longitudinal fluctuations prevalent ARGs (cfxA, tetM ermB) resistomes non-travelling volunteers. addition, assessed prevalence acquirable (blaCTX-M, qnrB, qnrS, vanA vanB) over time. Faecal samples from 23 participants were collected at baseline after 2 4 weeks. DNA was isolated, quantification performed by quantitative polymerase chain reaction adjusting for total amount bacterial 16S rDNA. qnrS not detected any samples, while vanB non-enterococcal origin qnrB 73.9% 5.7%, respectively. ß-lactamase encoding blaCTX-M 17.4% participants. results compared with previous data 122 travellers. acquisitions travellers rare individuals weeks follow-up, supporting hypothesis However, median -1.04- 1.04-fold changes 100% cfxA, ermB, which did differ those found Thus, common occur independent travel behaviour.