Selected neurotrophins, neuropeptides, and cytokines: developmental trajectory and concentrations in neonatal blood of children with autism or Down syndrome

• DOI: 10.1016/j.ijdevneu.2005.10.003
• Publication Date: 2005-11-15T12:14:49Z
• Authors: Phillip G. Nelson; Thea Kuddo; Eun Young Song; James M. Dambrosia; Shawn Kohler; Gowri Satyanarayana; Cassandra VanDunk; Judith K. Grether; Karin B. Nelson
• Keywords: Clinical Significance

Using a double-antibody immunoaffinity assay (Luminex) and ELISA technology, we measured concentrations of certain neurotrophins, neuropeptides, cytokines in pooled samples (one to three subjects per sample) eluted from archived neonatal blood children with later-diagnosed autism, Down syndrome, very preterm birth, or term control infants. We also analytes healthy adult controls. Case status for infant was ascertained by retrospective review service agency medical records. observed inhibitory substances eluates bloodspots, especially marked measurement BDNF. Concentrations differed age: BDNF rose markedly age, while NT-3 NT-4/5 were lower adults than newborn IL-8 higher infants, term, adults. Considered diagnostic group, total protein syndrome either autism subjects. In infants levels controls, whether not corrected protein; CGRP VIP higher. autistic subjects, significantly controls an increase approached statistical significance. correlated but Some these results differ earlier findings using single-antibody recycling chromatography (RIC) system. discuss interrelationships VIP, their potential relevance features the neuropathology syndrome.