Comparative evaluation of the new xTAG GPP multiplex assay in the laboratory diagnosis of acute gastroenteritis. Clinical assessment and potential application from a multicentre Italian study

• DOI: 10.1016/j.ijid.2015.02.011
• Publication Date: 2015-03-05T04:49:49Z
• Authors: C. Vocale; S.G. Rimoldi; C. Pagani; R. Grande; F. Pedna; M. Arghittu; G. Lunghi; A. Maraschini; M.R. Gismondo; M.P. Landini; E. Torresani; F. Topin; V. Sambri
• Keywords: Microbiology (medical); Adult; Diarrhea; Male; 0301 basic medicine; Infectious and parasitic diseases; RC109-216; Sensitivity and Specificity; Immunoenzyme Techniques; Feces; 03 medical and health sciences; Molecular assay; Animals; Humans; Parasites; Gastrointestinal pathogens; Acute diarrhoea; Molecular assay; Laboratory diagnosis; Multiplex PCR; Acute diarrhoea; Gastrointestinal pathogens; Laboratory diagnosis; Molecular assay; Multiplex PCR; Bacteria; Multiplex PCR; Middle Aged; Gastroenteritis; 3. Good health; Infectious Diseases; Italy; Molecular Diagnostic Techniques; Laboratory diagnosis; Viruses; Gastrointestinal pathogens; Female; Acute diarrhoea; Multiplex Polymerase Chain Reaction

Gastroenteritis caused by a single pathogen or multiple pathogens remains a major diagnostic challenge for the laboratory. The treatment of diarrhoea is based on microbiological results. Diagnosis is achieved using different laboratory techniques that have variable sensitivity and specificity. xTAG GPP is a new multiplex PCR assay that simultaneously detects 15 different pathogens responsible for diarrhoea. The results of the first multicentre study in Italy to evaluate the potential clinical application of the GPP assay in the laboratory diagnosis of diarrhoea are reported here.Faeces specimens (N=664) from hospitalized patients were tested with the GPP assay using a Luminex 200 instrument. All specimens were run using comparator methods following a routine algorithm: culture for bacteria, enzyme immunoassay and PCR for viruses, and microscopy for parasites.Of the samples tested with the GPP, 53.61% (356/664) gave positive results, as compared to 45.33% by routine testing. Of the positive specimens, 34.55% showed the presence of genomic DNA from multiple pathogens. The Luminex method showed an increase in the percentage of positivity of 8.28%.The GPP assay can be considered a helpful tool for the detection of gastrointestinal pathogens, with a hands-on time of 5h; it provides accurate data for the clinical management of hospitalized patients and for epidemiological surveillance.