WarmStart colorimetric loop-mediated isothermal amplification for the one-tube, contamination-free and visualization detection of Shigella flexneri

• DOI: 10.1016/j.ijid.2021.09.015
• Publication Date: 2021-09-10T15:11:08Z
• Authors: Yaoqiang Shi; Min Xu; Xiaoqiong Duan; Shilin Li; Jia-wei Ding; Limin Chen
• Keywords: Serial dilution

Shigella flexneri (S. flexneri) is prevalent worldwide and the most common in many countries, causing highly contagious diarrhea, which seriously threatens public health. This study aimed to develop a colorimetric loop-mediated isothermal amplification (LAMP) for rapid, accurate, visualization detection of S. flexneri.According screened specific genes flexneri, three groups LAMP primers were designed evaluated, reaction volume was optimized. The specificity validated by 20 96 non-S. clinical isolates. In addition, sensitivity developed assay evaluated serial 10-fold dilutions plasmid DNA.A based on hypothetical protein gene (Accession: AE014073 Region: 4170556.4171068). method had good detecting enabled within 30 minutes, with limit 7*10° copies/μL. results could be easily identified color.This used rapid accurate diagnosis infection, especially remote hospitals laboratories under-equipped medical facilities, situations where an urgent needed.