Molecular epidemiology and antimicrobial resistance of Clostridioides difficile in Germany, 2014–2019

• DOI: 10.1016/j.ijmm.2021.151507
• Publication Date: 2021-04-20T20:42:52Z
• Authors: Ahmed Mohamed Mos...; Zia Ul Habib Bajwa; Alexander Halfmann; Alexander Mellmann; Anna Nimmesgern; Lena Margardt; Markus Bischoff; Lutz von Müller; Barbara Gärtner; Fabian K. Berger
• Keywords: Ribotyping; Molecular Epidemiology

Clostridioides difficile is a Gram positive spore-forming rod and mainly responsible for nosocomial diarrhea in developed nations. Molecular antimicrobial surveillance important monitoring the strain composition including genotypes of high epidemiological importance such as ribotype 027 (RT027) corresponding resistance patterns. 1535 isolates obtained from samples sent between 2014 2019 to German National Reference Center (NRC) diagnostic reasons (NRC set), 1143 Tertiary Care University Saarland, Germany (non-NRC were evaluated using antibiotic susceptibility testing ribotyping. In NRC set, RT027 overtook RT001, main RT found preceding studies, dominated with 36.2%, followed by RT001 (13.3%), RT014 (8.5%). Of note, since 2016 constant decrease could be noticed. non-NRC set large diversity was present (18%) (8.9%) being most prevalent. samples, towards metronidazole, vancomycin, moxifloxacin, clarithromycin rifampicin 2.7%, 0%, 57.1%, 53.2% 19.2%, respectively. Metronidazole almost exclusively isolates. Rifampicin also observed predominantly RT027, constituting an four-fold increase, when compared preceeding studies this region. conclusion these data demonstrate that driver metronidazole resistance, underlining continuous efforts.