Forward genetic analysis of monensin and diclazuril resistance in Eimeria tenella

• DOI: 10.1016/j.ijpddr.2023.05.002
• Publication Date: 2023-05-24T16:07:56Z
• Authors: Hongtao Zhang; Lei Zhang; Ganglin Ren; Hongbin Si; Xingju Song; Xianyong Liu; Xun Suo; Dandan Hu
• Keywords: Coccidia; Monensin; Nonsynonymous substitution

Worldwide distributed coccidiosis is caused by infection of both Eimeria species and Cystoisospora in the host intestine causes huge economic losses to livestock industry, especially poultry industry. The control such diseases relies mainly on chemoprophylaxis with anticoccidials, which has led a very common drug resistance this field. However, genetic mechanisms underlying many anticoccidial drugs remain unknown. In study, strains E. tenella resistant 250 mg/kg monensin were generated characterized. Forward approaches based pooled genome sequencing, including experimental evolution linkage group selection, used locate candidate targets responsible for diclazuril tenella. A total 16 nonsynonymous mutants protein-coding genes identified monensin-resistant strains, two genomic regions strong selection signals also detected diclazuril-resistant strains. Our study reveals characterization species, provides important information that contributes understanding molecular mechanism coccidia.