Characterization of the complete mitochondrial genomes of Diplodiscus japonicus and Diplodiscus mehari (Trematoda: Diplodiscidae): Comparison with the members of the superfamily Paramphistomoidea and phylogenetic implication

• DOI: 10.1016/j.ijppaw.2022.07.009
• Publication Date: 2022-08-05T03:46:55Z
• Authors: Qi An; Yang-Yuan Qiu; Yan Lou; Yan Jiang; Hong-Yu Qiu; Zhong-Huai Zhang; Ben Li; Ai-Hui Zhang; Wei Wei; Ying-Yu Chen; Jun-Feng Gao; Chun-Ren Wang
• Keywords: Stop codon; Monophyly

Diplodiscus japonicus and mehari (Trematoda: Diplodiscidae) are two important parasites in wood frogs, which have large infection rates essential importance of ecology, economy society. In this study, the complete mitochondrial (mt) genomes D. were sequenced, then compared with other related trematodes superfamily Paramphistomoidea. The circular mt sequence 14,210 bp 14,179 length, respectively. Both comprised 36 functional subunits, consisting 12 protein-coding genes (PCGs), ribosomal RNA (rRNA) genes, 22 transfer (tRNA) one non-coding region. transcribed same direction, gene arrangements identical to those Paramphistomoidea trematodes. PCGs, GTG was most common initiation codon, whereas TAG termination codon. All tRNAs had a typical cloverleaf structure except tRNA Ser1. A comparison trematode suggested that cox1 longest these Phylogenetic analyses revealed formed monophyletic branch, Paramphistomidae Gastrothylacidae more closely than Diplodiscidae. And further analysis Pronocephalata branch found flukes parasitic amphibians (frogs) group, from ruminants (cattle, sheep, ect) another group. Our study demonstrated sequencing mehari, will provide significant molecular resources for studies taxonomy, population genetics systematics.