Anti-inflammatory potential of Antrodia Camphorata through inhibition of iNOS, COX-2 and cytokines via the NF-κB pathway
0301 basic medicine
Cyclooxygenase 2 Inhibitors
Dose-Response Relationship, Drug
Macrophages
Anti-Inflammatory Agents
Intracellular Signaling Peptides and Proteins
NF-kappa B
Nitric Oxide Synthase Type II
Proteins
Nitric Oxide
Dinoprostone
Cell Line
3. Good health
Mice
03 medical and health sciences
Gene Expression Regulation
Cyclooxygenase 2
Animals
Cytokines
Polyporales
Reactive Oxygen Species
Drugs, Chinese Herbal
Interleukin-1
DOI:
10.1016/j.intimp.2005.06.013
Publication Date:
2005-07-19T14:39:28Z
AUTHORS (8)
ABSTRACT
Antrodia camphorata (A. camphorata), well known in Taiwan as a traditional Chinese medicine, has been shown to exhibit antioxidant and anticancer effects. In the present study, therefore, we have examined the effects of the fermented culture broth of A. camphorata (25-100 microg/ml) in terms of lipopolysaccharide (LPS)-induced nitric oxide (NO) and prostaglandin E2 (PGE2) production, and inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein expression in RAW 264.7 macrophages. Our results indicate concentration-dependent A. camphorata inhibition of LPS-induced NO and PGE2 production, without appreciable cytotoxicity on the RAW 264.7 cells. A. camphorata also attenuates the production of LPS-induced tumor necrosis factor (TNF-alpha) and interleukin (IL)-1beta. Furthermore, A. camphorata blocks the IkappaB-alpha degradation induced by LPS. These results indicate that A. camphorata inhibits LPS induction of cytokine, iNOS and COX-2 expression by blocking NF-kappaB activation. Therefore, we report the first confirmation of the anti-inflammatory potential of this traditionally employed herbal medicine in vitro.
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