Increased production of nitric oxide by neutrophils from patients with chronic granulomatous disease on interferon-gamma treatment

• DOI: 10.1016/j.intimp.2012.01.016
• Publication Date: 2012-02-11T09:18:10Z
• Authors: Fariba Naderi beni; Fatemeh Fattahi; Abbas Mirshafiey; Mohammad Ansari; Monireh Mohsenzad...; Masoud Movahedi; Zahra Pourpak; Mostafa Moin
• Keywords: EXPRESSION; Adult; Male; 0301 basic medicine; Nitric oxide (NO); Adolescent; Neutrophils; Granulomatous Disease, Chronic; Nitric Oxide; PHAGOCYTOSIS; MECHANISMS; SUPEROXIDE; Interferon-gamma; Young Adult; 03 medical and health sciences; Humans; Child; Interferon gamma (IFN-gamma); Cells, Cultured; Nitrites; MONONUCLEAR-CELLS; RELEASE; Trimethoprim-sulfamethoxazole (TMP-SMX); IFN-GAMMA; Nitrates; TRIMETHOPRIM-SULFAMETHOXAZOLE; Infant; POLYMORPHONUCLEAR LEUKOCYTES; 3. Good health; Chronic granulomatous disease (CGD); INFECTIONS; Child, Preschool; Female

Chronic granulomatous disease (CGD) is a rare immunodeficiency disorder in which phagocytic leukocytes fail to generate superoxide (O(2)(-)) and antimicrobial oxidants. The therapeutic validity of interferon-gamma (IFN-γ) has been well established in CGD patients but its underlying mechanisms remain poorly understood. One probable mechanism has been suggested to be modulation of nitric oxide (NO) release from phagocytic cells. Herein, we investigated NO production from neutrophil cells in CGD patients on treatment with IFN-γ in vivo and in vitro. We measured NO levels in sera from 19 CGD patients (group I: 7 patients treated with TMP-SMX, group II: 12 patients treated with TMP-SMX and IFN-γ simultaneously) and healthy control individuals (8 cases). We also measured NO production from neutrophils in both patients groups as well as in control group after adding 100 U IFN-γ in vitro. Our results showed that there was a significant difference between the groups in the NO levels of serum; patients who received IFN-γ had significantly higher amount of NO than the other groups. Besides, NO levels increased significantly after adding 100 U IFN-γ in vitro in three studied groups, considerably in the patients on treatment with IFN-γ. As a brief conclusion, the effect of IFN-γ in increasing NO production is obvious. This could be an explanation for the therapeutic effect of IFN-γ in patients with CGD as NO acts as a bactericidal agent and plays a role in host defense mechanism instead of O(2)(-).