Anthraquinone derivative O,O′-bis-(3′-iodopropyl)-1,4-dihidroxyanthraquinone modulates immune response and improves experimental autoimmune encephalomyelitis

• DOI: 10.1016/j.intimp.2012.06.013
• Publication Date: 2012-06-29T04:30:21Z
• Authors: Caio C.S. Alves; Sandra B.R. Castro; Cristiane F. Costa; Alyria T. Dias; Chrystian J. Alves; Michele F. Rodrigues; Henrique C. Teixeira; Mauro V. Almeida; Ana Paula Ferreira
• Keywords: 0301 basic medicine; Encephalomyelitis, Autoimmune, Experimental; Multiple Sclerosis; Cell Survival; Immunology; Cell Line; Multiple sclerosis; Immunomodulation; Mice; 03 medical and health sciences; Cell Movement; Immunology and Allergy; Animals; Humans; Mitoxantrone analog; Pharmacology; EAE; Immunity; 3. Good health; Mice, Inbred C57BL; Spinal Cord; Macrophages, Peritoneal; Cytokines; Female; Chemokines; Inflammation Mediators; Mitoxantrone

The present study investigated the effects of the anthraquinone derivative (O,O'-bis-(3'-iodopropyl)-1,4-dihidroxyanthraquinone - DIPDHAQ), mitoxantrone analog, in an experimental autoimmune encephalomyelitis (EAE) model. The results showed that DIPDHAQ treatment improved the clinical signs of the disease (n=10; vehicle: 3.8 ± 0.3; DIPDHAQ: 1.4 ± 0.9). The improvement was associated with a decrease of inflammatory cells, demyelination, IL-17, IFN-γ, IL-12p40, IL-6, TGF-β, CCL5 and CCL20 levels in the spinal cord. DIPDHAQ presented a low cytotoxicity when in vitro assays were performed. Therefore, the findings suggest a major role for DIPDHAQ in multiple sclerosis, disease characterized as an autoimmune inflammatory disorder against myelin proteins of the brain and spinal cord. The attenuation of inflammation and consequently improvement of clinical signs, involving a decrease of pro-inflammatory cytokines and the low cytotoxicity of DIPDHAQ, suggest that this compound could be used as an alternative treatment for autoimmune diseases in the central nervous system.