RBM47 as a potential therapeutic target for thyroid-associated ophthalmopathy

• DOI: 10.1016/j.intimp.2024.113955
• Publication Date: 2025-01-01T18:58:52Z
• Authors: Ru Zhu; Fei Chen; Bo-Wen Wang; Ying Jin; Jun-Jie Yang; Bing-Jie Shi; Yu-Ting Chen; Fa-Gang Jiang; Xing-Hua Wang
• Keywords: Graves' ophthalmopathy

RNA-binding motif 47 (RBM47) is a recently identified protein involved in early vertebrate development, immune homeostasis, and cancer development. This study examined the biological functions of RBM47 thyroid-associated ophthalmopathy (TAO). Orbital fibroblasts (OFs) were obtained from control (n = 6) TAO groups 6). Protein gene expression samples investigated using immunohistochemistry, western blotting (WB), RT-PCR. OFs with knockdown established small interfering RNA. Subsequently, Oil Red O staining, WB, RT-PCR performed to assess adipogenesis OFs. The IL-1β-induced proinflammatory molecules hyaluronan (HA) was determined enzyme-linked immunosorbent assay Moreover, TGF-β-induced fibrosis evaluated scratch assays, RT-PCR, WB. markedly increased orbital tissues individuals TAO. decreased OFs, downregulated levels insulin-like growth factor 1 receptor (IGF-1R), molecules, HA. Furthermore, low IGF-1R, which subsequently inhibited adipocyte differentiation by decreasing extracellular signal-regulated kinase signalling. These findings indicate that may be regulation adipogenesis, inflammation, HA production, fibrosis, highlighting its potential as therapeutic target for