Unveiling Molecular Recognition of Sialoglycans by Human Siglec-10

• DOI: 10.1016/j.isci.2020.101231
• Publication Date: 2020-06-02T15:49:20Z
• Authors: Rosa Ester Forgione; Cristina Di Carlu...; Juan Guzmán-Calde...; Rosa Gaglione; Filomena Battista; Fabrizio Chiodo; Yoshiyuki Manabe; Angela Arciello; Pompea Del Vecchio; Koichi Fukase; Antonio Molinaro; Sonsoles Martín-S...; Paul R. Crocker; Roberta Marchetti; Alba Silipo
• Keywords: SIGLEC; Molecular Recognition

Siglec-10 is an inhibitory I-type lectin selectively recognizing sialoglycans exposed on cell surfaces, involved in several patho-physiological processes. The key role plays the regulation of immune functions has made it a potential target for development immunotherapeutics against broad range diseases. However, crystal structure protein not been resolved time being and atomic description interactions with complex glycans previously unraveled. We present here first insights molecular mechanisms regulating interaction between naturally occurring sialoglycans. used combined spectroscopic, computational biophysical approaches to dissect glycans' epitope mapping conformation upon binding order afford 3D complexes. Our outcomes provide structural perspective rational design high-affinity ligands control receptor functionality.