Classifying Drosophila olfactory projection neuron boutons by quantitative analysis of electron microscopic reconstruction

• DOI: 10.1016/j.isci.2022.104180
• Publication Date: 2022-04-01T06:36:08Z
• Authors: Kai Yang; Tong Liu; Ze Wang; Jing Liu; Yuxinyao Shen; Xinyi Pan; Ruyi Wen; Haotian Xie; Zhaoxuan Ruan; Zixiao Tan; Yingying Chen; Aike Guo; He Liu; Hua Han; Zengru Di; Ke Zhang
• Keywords: Antennal lobe

In Drosophila melanogaster, olfactory projection neurons (PNs) convey odor information from the antenna lobe to higher brain regions. Recent transcriptomic studies reveal a large diversity of transcription factors, cell-surface molecules, neurotransmitter-coding, and neuropeptide-coding genes in PNs; however, their structural remains unknown. Herein, we achieved volumetric reconstruction 89 PN boutons under Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) quantitatively analyzed internal presynaptic active zones (PAZs) dense-core vesicles (DCVs). The ultrastructure-based cluster analysis reveals three morphological distinct bouton subtypes: complex boutons, unilobed simple boutons. contain most PAZs DCVs, which suggests that they are highest capability releasing neurotransmitters neuromodulators. By labeling subset FIB-SEM, found DCVs preferentially distributed certain GH146-positive subtypes. Our study demonstrates display morphology, may determine capacity