Pancreatic islet cell type–specific transcriptomic changes during pregnancy and postpartum

• DOI: 10.1016/j.isci.2023.106439
• Publication Date: 2023-03-17T21:14:19Z
• Authors: Jin-Yong Chung; Yongjie Ma; Dingguo Zhang; Hayden H. Bickerton; Eric Stokes; Sweta B. Patel; Hubert M. Tse; Joseph Feduska; Rob S. Welner; Ronadip R. Banerjee
• Keywords: Pregnancy; Science; Q; Transcriptomics; Molecular physiology

Highlights•ScRNA-seq of pancreatic islets comparing virgin, pregnant, and postpartum mice•Islet cell types exhibit unique transcriptomic responses in pregnancy postpartum•β-cells show induction immediate early genes (IEGs) postpartum•IEGs β-cells do not correlate with apoptosis may mediate survivalSummaryPancreatic β-cell mass expands during regresses the period conjunction dynamic metabolic demands on maternal glucose homeostasis. To understand transcriptional changes driving these adaptations other islet types, we performed single-cell RNA sequencing from late gestation, mice. We identified signatures to gestation β-cells, including AP-1 transcription factor subunits involved immediate-early response (IEGs). In addition, found postpartum-induced differed within each endocrine type, endothelial cells antigen-presenting islets. Together, our data reveal insights into type–specific responsible for by their resolution postpartum.Graphical abstract