Cutoff value of IC50 for drug sensitivity in patient-derived tumor organoids in colorectal cancer

• DOI: 10.1016/j.isci.2023.107116
• Publication Date: 2023-06-16T04:03:05Z
• Authors: Yuting Tang; Ting Wang; Yaowen Hu; Hongli Ji; Botao Yan; Xiarong Hu; Yunli Zeng; Yifan Hao; Weisong Xue; Zexin Chen; Jianqiang Lan; Yanan Wang; Haijun Deng; Chuxia Deng; Xiufeng Wu; Jun Yan
• Keywords: FOLFOX; Cut-off; IC50; Organoid

Patient-derived tumor organoids (PDTOs) have the potential to be used predict patient response chemotherapy. However, cutoff value of half-maximal inhibition concentration (IC50) for PDTO drug sensitivity has not been validated with clinical cohort data. We established PDTOs and performed a test in 277 samples from 242 CRC patients who received FOLFOX or XELOX After follow-up comparison final outcome results, optimal IC50 was 43.26 μmol/L. This test-defined could 75.36% sensitivity, 74.68% specificity, 75% accuracy. Moreover, this distinguished groups significant differences survival benefit. Our study is first define effectively distinguish chemosensitivity nonsensitivity benefits.