Integrated analysis of whole blood oxylipin and cytokine responses after bacterial, viral, and T cell stimulation reveals new immune networks

• DOI: 10.1016/j.isci.2023.107422
• Publication Date: 2023-07-18T06:18:38Z
• Authors: Etienne Villain; Aurélie Chanson; Malwina Mainka; Nadja Kampschulte; Pauline Le Faouder; Justine Bertrand-...; Marion Brandolini...; Bruno Charbit; Munyaradzi Musvosvi; Nicole Bilek; Thomas J. Scriba; Lluis Quintana-Murci; Nils Helge Schebb; Darragh Duffy; Cécile Gladine; Laurent Abel; Andres Alcover; Hugues Aschard; Philippe Bousso; Nollaig Bourke; Petter Brodin; Pierre Bruhns; Nadine Cerf-Bensu...; Ana Cumano; Christophe D’Enfert; Ludovic Deriano; Marie-Agnès Dillies; James Di Santo; Gérard Eberl; Jost Enninga; Jacques Fellay; Ivo Gomperts-Boneca; Milena Hasan; Gunilla Karlsson ...; Serge Hercberg; Molly A. Ingersoll; Olivier Lantz; Rose Anne Kenny; Mickaël Ménager; Hugo Mouquet; Cliona O'Farrelly; Etienne Patin; Sandra Pellegrini; Antonio Rausell; Frédéric Rieux-La...; Lars Rogge; Magnus Fontes; Anavaj Sakuntabhai; Olivier Schwartz; Benno Schwikowski; Spencer Shorte; Frédéric Tangy; Antoine Toubert; Mathilde Touvier; Marie-Noëlle Unge...; Christophe Zimmer; Matthew L. Albert; Darragh Duffy; Lluis Quintana-Murci
• Keywords: Oxylipin

Oxylipins are major immunomodulating mediators, yet studies of inflammation focus mainly on cytokines. Here, using a standardized whole-blood stimulation system, we characterized the oxylipin-driven inflammatory responses to various stimuli and their relationships with cytokine responses. We performed pilot study in 25 healthy individuals 6 different stimuli: 2 bacterial (LPS live BCG), viral (vaccine-grade poly I:C H1N1 attenuated influenza), an enterotoxin superantigen Null control. All induced strong production oxylipins but most importantly, bacterial, viral, T cell immune show distinct oxylipin signatures. Integration for each condition revealed new networks improving our understanding regulation. Finally, oxylipin-cytokine were compared patients active tuberculosis or latent infection. This BCG not LPS highlighting regulatory pathways further investigations.