Comprehensive characterization of patient-derived xenograft models of pediatric leukemia

• DOI: 10.1016/j.isci.2023.108171
• Publication Date: 2023-10-10T17:56:11Z
• Authors: Anna Rogojina; Laura J. Klesse; Erin Butler; Jiwoong Kim; He Zhang; Xue Xiao; Lei Guo; Qinbo Zhou; Taylor Hartshorne; Dawn Garcia; Korri Weldon; Trevor Holland; Abhik Bandyopadhyay; Luz Perez Prado; Shidan Wang; Donghan M. Yang; Anne-Marie Langevan; Yi Zou; Allison C. Grimes; Chatchawin Assanasen; Vinod Gidvani-Diaz; Siyuan Zheng; Zhao Lai; Yidong Chen; Yang Xie; Gail E. Tomlinson; Stephen X. Skapek; Raushan T. Kurmas...; Peter J. Houghton; Lin Xu
• Keywords: Science; Q; Omics; Transcriptomics; Article; 3. Good health; Cancer

Patient-derived xenografts (PDX) remain valuable models for understanding the biology and developing novel therapeutics. To expand current PDX of childhood leukemia, we have developed new from Hispanic patients, a subgroup with poorer overall outcome. Of 117 primary leukemia samples obtained, successful engraftment serial passage in mice were achieved 82 (70%). patient engrafted at rate (51/73, 70%) that was similar to non-Hispanic (31/45, 70%). With algorithm remove mouse contamination multi-omics datasets including methylation data, found faithfully reflected somatic mutations, copy-number alterations, RNA expression, gene fusions, whole-genome patterns, immunophenotypes tumor (PT) first 50 reported here. This cohort characterized leukemias represents resource germline DNA sequencing has allowed unambiguous determination mutations both PT PDX.