AMPKα2 is a skeletal muscle stem cell intrinsic regulator of myonuclear accretion

• DOI: 10.1016/j.isci.2023.108343
• Publication Date: 2023-10-27T00:37:04Z
• Authors: Anita Kneppers; Sabrina Ben Larbi; Marine Theret; Audrey Saugues; Carole Dabadie; Linda Gsaier; Arnaud Ferry; Philipp Rhein; Julien Gondin; Kei Sakamoto; Rémi Mounier
• Keywords: Stem cells research; Molecular biology experimental approach; Science; Q; [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]; Specialized functions of cells; Molecular mechanism of behavior; Article; cell Biology

Due to the post-mitotic nature of skeletal muscle fibers, adult maintenance relies on dedicated stem cells (MuSCs). In most physiological contexts, MuSCs support myofiber homeostasis by contributing myonuclear accretion, which requires a coordination cell-type specific events between and MuSCs. Here, we addressed role kinase AMPKα2 in these supporting accretion. We demonstrate that deletion impairs regeneration. Through vitro assessments MuSC myogenic fate EdU-based cell tracing, reveal MuSC-specific regulation is mediated phosphorylation non-metabolic substrate BAIAP2. Similar tracing vivo shows knockout mice have lower rate accretion during regeneration, decreases response contraction. Together, this demonstrates MuSC-intrinsic regulator