GPR162 is a beta cell CART receptor

• DOI: 10.1016/j.isci.2023.108416
• Publication Date: 2023-11-10T12:54:12Z
• Authors: Andreas Lindqvist; Mia Abels; Liliya Shcherbina; Mtakai Ngara; Dmytro Kryvokhyzha; Sabrina Chriett; Matteo Riva; Abul Fajul; Mohammad Barghouth; Cheng Luan; Lena Eliasson; Olav Larsen; Mette M. Rosenkilde; Enming Zhang; Erik Renström; Nils Wierup
• Keywords: Cart; Pancreatic Islets

Cocaine and amphetamine-regulated transcript (CART) is expressed in pancreatic islet cells neuronal elements. We have previously established insulinotropic actions of CART human rodent islets. The receptor for the beta unidentified. used RNA sequencing Cartpt knockdown (KD) INS-1 832/13 identified GPR162 as most Cartpt-regulated receptor. therefore tested if mediates effects cells. Binding to was using proximity ligation assay, radioactive binding, co-immunoprecipitation, KD Gpr162 mRNA caused reduced binding. had blunted CARTp-induced exocytosis, insulin secretion. Furthermore, we a hitherto undescribed GPR162-dependent role regulator cytoskeletal arrangement. Thus, our findings provide mechanistic insight into effect on secretion show that