Demethylase FTO-mediated m6A modification of SIK1 modulates placental cytotrophoblast syncytialization in type 2 diabetes mellitus

• DOI: 10.1016/j.isci.2024.109900
• Publication Date: 2024-05-04T09:53:45Z
• Authors: Jie Ning; Jie Yan; Shuxian Wang; Zifeng Cui; Yiwei Xue; Juan Juan; Huixia Yang
• Keywords: Cytotrophoblast; Demethylase; Immunoprecipitation; Trophoblast; Epigenome; RNA methylation

Type 2 diabetes mellitus (T2DM) represents a common complication during pregnancy that affects fetoplacental development. We demonstrated the existence of impaired trophoblast syncytialization under hyperglycemic conditions. However, exact mechanism remains unknown. RNA N6-methyladenosine (m6A) is an emerging regulatory mRNA and participates in various biological processes. described global m6A modification pattern T2DM placenta by combined analysis methylated immunoprecipitation sequencing (MeRIP-Seq) (RNA-Seq). Both expression