In-depth functional analysis of BRD9 in fetal hematopoiesis reveals context-dependent roles

• DOI: 10.1016/j.isci.2025.112010
• Publication Date: 2025-02-12T17:22:24Z
• Authors: Yifan Zhang; Masaki Nomura; Koutarou Nishimura; Weijia Zang; Yui Koike; Muran Xiao; Hiromi Ito; Miki Fukumoto; Atsushi Tanaka; Yumi Aoyama; Wataru Saika; Chihiro Hasegawa; Hiromi Yamazaki; Akifumi Takaori-K...; Daichi Inoue
• Keywords: Cell biology; Molecular biology; Science; Q; Omics

The hierarchical organization of hematopoietic stem cells (HSCs) governing adult hematopoiesis has been extensively investigated. However, the dynamic epigenomic transition from fetal to remains incompletely understood, particularly regarding involvement epigenetic factors. In this study, we investigate roles BRD9, an essential component non-canonical BAF (ncBAF) complex known govern fate HSCs, in hematopoiesis. Consistent with observations hematopoiesis, BRD9 loss impairs HSC stemness and disturbs erythroid maturation. Intriguingly, impact on myeloid lineage was discrepant: inhibited promoted differentiation models, respectively. Through comprehensive transcriptomic analysis, elucidate differential a context- lineage-dependent manner. Our data uncover how BRD9/ncBAF modulates transcription stage-specific manner, providing deeper insights into regulation underlying