CXCR4+ PD-L1+ neutrophils are increased in non-survived septic mice

• DOI: 10.1016/j.isci.2025.112083
• Publication Date: 2025-02-22T16:35:33Z
• Authors: Guilherme Cesar M...; Maísa de Oliveira...; Antonio Edson Roc...; Kalil Alves de Lima; Paula Barbim Donate; Cleyson da Cruz O...; Anderson dos Sant...; Victor Costa; Daniele Carvalho ...; Luis Eduardo Alve...; Amanda Curto Tavares; André Nicolau Aqu...; Helder Takashi Im...; Thiago Mattar Cunha; José Carlos Alves...; Fernando Queiroz ...

The dysregulated host response to infections can lead sepsis, a complex disease characterized by spectrum of clinical phenotypes. Using scRNA-seq, we analyzed the immune cell survived and non-survived CLP-septic mice gain insights into immunological mechanisms which neutrophils contribute hyperinflammatory phenotype. Our findings reveal that exhibit increased frequencies immature CXCR4+ PD-L1+ in bloodstream, accompanied an accumulation trafficking-specific lungs. IFN-gamma LPS promote PD-L1 expression on activation profile associated with inflammation organ damage. Notably, abrogating reduced susceptibility CLP-sepsis diminished frequency. This study provides profiles worsening CLP-sepsis, population highlighted here represents promising target for therapeutic modulation sepsis