Green seaweed Caulerpa racemosa - Chemical constituents, cytotoxicity in breast cancer cells and molecular docking simulation

• DOI: 10.1016/j.jafr.2023.100621
• Publication Date: 2023-05-13T06:26:29Z
• Authors: Grace Sanger; Djuhria Wonggo; Nurmelita Taher; Verly Dotulong; Aurielle Annalici...; Happy Kurnia Perm...; Sidik Maulana; Fahrul Nurkolis; Apollinaire Tsopmo; Bonglee Kim
• Keywords: Phytochemical; MTT assay

Marine and terrestrial organisms are rich in chemical compounds with medicinal pharmacological properties, including antitumor agents for chemoprevention. Caulerpa racemosa, a marine species, is potential source of novel therapeutic human cancer. This study aimed to determine the anticancer activity C. racemosa extracts breast cancer cells, identify compounds, mechanism using computational models. Seaweed (C. racemosa) was taken from North Sulawesi, Indonesia; Followed by authentication identification according previously published protocol extracted three different solvent: hexane, ethyl acetate, ethanol. were evaluated cytotoxicity against MCF-7 cells MTT (3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide) assay. Antioxidant activities assessed based on free radical scavenging (2,2-diphenyl-1-picrylhydrazyl (DPPH)) ferric-reducing antioxidant power (FRAP) assays. The phytochemical constituents identified liquid chromatography-electrospray ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF-MS) system. interaction epidermal growth factor 2 (HER2) protein achieved molecular docking PyRx-vina application protein-ligand complex visualization. hexane extract exhibited highest followed acetate ethanol (IC50 23.7 ± 2.0; 66.7 5.8 182.7 14.3 μg/mL). best sample DPPH 21.5 2.0 μg/mL) while most active FRAP value (14.5 1.3 μg gallic acid equivalent/g). Data LC-ESI-QTOF-MS allowed 21 compounds. showed that 12 could prevent tumors acting as inhibitors anti-apoptotic HER2 protein. has chemoprevention through its capacity inhibition More studies needed investigate efficacy