Single garlic oil modulates T cells activation and proinflammatory cytokine in mice with high fat diet

• DOI: 10.1016/j.jaim.2020.06.009
• Publication Date: 2020-08-12T05:43:42Z
• Authors: Sri Rahayu Lestari; Mochammad Fitri A...; Yuyun Ika Christina; Muhaimin Rifa’i
• Keywords: 0301 basic medicine; 0303 health sciences; 03 medical and health sciences; High-fat diet; T-cells; Original Research Article (Experimental); RZ409.7-999; Single garlic; Miscellaneous systems and treatments; 3. Good health

Hyperlipidemia triggers atherosclerosis by involving immune cells, such as T-cells. T-cells plays a role in worsening conditions during a high-fat diet (HFD).The research aimed to analyze the role of single garlic oil (SGO) on T-cells activation and its proinflammatory cytokine expression in HFD mice.Mice were divided into six groups: ND (normal diet); HFD (high-fat diet without treatment); HFD + Simv (HFD + simvastatin 2.6 mg/kg body weight); and HFD + SGO 1-3 (high-fat diet + single garlic oil in a dose of 12.5, 25, and 50 mg/kg body weight), respectively. Treatments were orally given every day for 45 days. At the end of treatments, lymphocytes were isolated from mice spleen. The relative number of T-cells and proinflammatory cytokines were measured using flow-cytometry and analyzed using one-way ANOVA (p < 0.05).Our result indicated that HFD mice had lower naive T cells (CD4+CD62L+) than normal mice (p < 0.05). SGO treatment in HFD mice increased the relative number of naïve T cells. HFD treatment increased the expression of TNF-α and IFN-γ through NF-κB expression. Furthermore, SGO treatment improved the expression of TNF-α and IFN-γ.Our study suggests that SGO could act as a promising prospect for therapy to improve chronic inflammation in a HFD.