P1‐029: Effect of cortical placement of luteinizing hormone on Aβ metabolism in guinea pigs

• DOI: 10.1016/j.jalz.2009.04.032
• Publication Date: 2009-06-25T11:17:42Z
• Authors: Linda Kurnia Wijaya; Eka Julianta Wahj...; Kevin Taddei; Georgia Martins; Matthew Howard; Kristyn Bates; Satvinder Dhaliwal; Giuseppe Verdile; Ralph Martins
• Keywords: 03 medical and health sciences; 0302 clinical medicine

The rise in luteinizing hormone (LH) concentration during senescence has been associated with increased risk of developing Alzheimer's disease (AD). LH shown to modulate the amyloidogenic processing amyloid precursor protein (APP). receptor (LHR) localised limbic system, particular hippocampus, area that is devastated AD. Therefore, we administered either or placebo containing slow-release pellets within cortex castrated guinea pigs, where amyloid-beta (Aβ) sequence identical human, investigate direct effect on Aβ metabolism vivo. Twenty-four pigs (8-months old, ~500 gram) were divided into 4 groups (n=6) and (n=12) by cortical placement. Animals then sacrificed at 14 28 days post administration pellets. Intact animals (n=23) day 0 used as a baseline control. Tissues (plasma, cerebrospinal fluid (CSF) brain) collected baseline, days-post administration.Aβ levels measured using sensitive ELISA technique other AD-related proteins, such APP, APP C-terminal fragment (CTF) secreted alpha (αAPPs) western blot. We observed significant increase CSF brain compared intact control group (p<0.05). also αAPPs (p<0.05), which may indicate delayed process recovery reported be neuroprotective. This study for first time effects presence human CNS accumulation. provides suitable vivo model further examine mechanisms action underlie regulation processing.