Circulating immune profile in granulomatosis with polyangiitis reveals distinct patterns related to disease activity

• DOI: 10.1016/j.jaut.2024.103236
• Publication Date: 2024-04-30T21:41:04Z
• Authors: C.G. Bonasia; N. Inrueangsri; T. Bijma; K.P. Mennega; R. Wilbrink; S. Arends; W.H. Abdulahad; N.A. Bos; A. Rutgers; P. Heeringa
• Keywords: Granulomatosis with polyangiitis

Granulomatosis with polyangiitis (GPA) is an autoimmune disorder characterized by recurrent relapses that can cause severe tissue damage and life-threatening organ dysfunction. Multiple immune cells cytokines/chemokines are involved in the different stages of disease. Immune profiling patients may be useful for tracking disease activity, however, reliable signatures GPA activity lacking. In this study, we examined circulating profiles during active remission states to identify potential patterns associated activity. The distribution phenotypic characteristics major cells, cytokines/chemokines, were studied on cryopreserved peripheral blood mononuclear from (active, n = 20; remission, 20) healthy controls (n leveraging a 40-color optimized multicolor immunofluorescence panel (OMIP-69) serum using 46-plex Luminex multiplex assay, respectively. Deep phenotyping uncovered distinct composition most significant findings emerging within monocyte compartment. Our detailed analysis revealed diversity beyond conventional subsets. We identified eight classical populations, two intermediate one non-classical population. Notably, had higher frequency CD45RA+CCR5+CCR6−CCR7+/lowCD127−HLA-DR+CD2− monocytes lower CD45RA−CCR5-/lowCCR6−CCR7−CD127−HLA-DR+CD2+/− monocytes, which both strongly correlated Furthermore, levels CXCL1, CXCL2, CCL20, all linked biology, elevated These shed light profile lead signature assessing Monocytes particular further as markers monitoring GPA.