The ephrin receptor EphB2 regulates the connectivity and activity of enteric neurons

• DOI: 10.1016/j.jbc.2021.101300
• Publication Date: 2021-10-11T13:58:50Z
• Authors: Raphael Bodin; Vincent Paillé; Thibauld Oullier; Tony Durand; Philippe Aubert; Catherine Le Berr...; Philippe Hulin; Michel Neunlist; Moustapha Cissé
• Keywords: Enteric Nervous System; Synapsin I; Biological neural network

Highly organized circuits of enteric neurons are required for the regulation gastrointestinal functions, such as peristaltism or migrating motor complex. However, factors and molecular mechanisms that regulate connectivity their assembly into functional neuronal networks largely unknown. A better understanding by which neurotrophic this neuron circuitry is paramount to nervous system (ENS) physiology. EphB2, a receptor tyrosine kinase, essential plasticity in brain, but so far its presence function ENS remain unexplored. Here we report EphB2 expressed preferentially relative glial cells throughout gut rats. We show primary neurons, activation natural ligand ephrinB2 engages ERK signaling pathways. Long-term with decreases expression reduces without affecting density, ganglionic fiber bundles, overall morphology. This highlighted loss markers synapsin I, PSD95, synaptophysin, decrease spontaneous miniature synaptic currents. 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