Mechanisms by which small molecules of diverse chemotypes arrest Sec14 lipid transfer activity

• DOI: 10.1016/j.jbc.2022.102861
• Publication Date: 2023-01-02T23:48:54Z
• Authors: Xiao-Ru Chen; Lokendra Poudel; Zebin Hong; Philipp Johnen; Sachin Katti; Ashutosh Tripathi; Aaron H. Nile; Savana M. Green; Danish Khan; Gabriel Schaaf; Fulvia Bono; Vytas A. Bankaitis; Tatyana I. Igumenova
• Keywords: 0301 basic medicine; 570; 03 medical and health sciences; Saccharomyces cerevisiae Proteins; Antifungal Agents; Drug Design; Biological Transport; Saccharomyces cerevisiae; Phospholipid Transfer Proteins; Phosphatidylinositols; Research Article; Signal Transduction

Phosphatidylinositol (PtdIns) transfer proteins (PITPs) enhance the activities of PtdIns 4-OH kinases that generate signaling pools of PtdIns-4-phosphate. In that capacity, PITPs serve as key regulators of lipid signaling in eukaryotic cells. Although the PITP phospholipid exchange cycle is the engine that stimulates PtdIns 4-OH kinase activities, the underlying mechanism is not understood. Herein, we apply an integrative structural biology approach to investigate interactions of the yeast PITP Sec14 with small-molecule inhibitors (SMIs) of its phospholipid exchange cycle. Using a combination of X-ray crystallography, solution NMR spectroscopy, and atomistic MD simulations, we dissect how SMIs compete with native Sec14 phospholipid ligands and arrest phospholipid exchange. Moreover, as Sec14 PITPs represent new targets for the development of next-generation antifungal drugs, the structures of Sec14 bound to SMIs of diverse chemotypes reported in this study will provide critical information required for future structure-based design of next-generation lead compounds directed against Sec14 PITPs of virulent fungi.