Abstract 1601 Use of Interactive Progress Curves in an OER to Teach Kinetics in Biochemistry

• DOI: 10.1016/j.jbc.2024.105924
• Publication Date: 2024-03-25T21:43:02Z
• Authors: Henry Jakubowski; Henry Agnew; Bartholomew Jardine; Herbert Sauro

Enzyme kinetics is challenging for students to understand and apply. Why? Perhaps it's because we teach it in isolation rarely revisit a class or throughout the biology chemistry curricula. It also requires integrate mathematical, structural, mechanistic understandings, tall order requiring cognitive processing multiple fields. Students generally conservation of mass, length, volume by about age 11, but derivative quantities take longer. Examples include density (m/V, 15) both concentration (mol/L) velocity (m/s) high school. Michaelis-Menten uses more nuanced quantity, initial (mol/L/s) at time = 0). We then focus almost exclusively on plots v0 vs [S] +/- effectors where not an obvious parameter. No wonder struggle with enzyme kinetics. To make mathematics understandable, have developed series interactive progress curves (concentration time) models Fundamentals Biochemistry (FOB). This online free, multivolume biochemistry textbook part LibreText collections. Users can move sliders change constants concentrations observe immediate changes reactants products time. These are intuitive (for example, reactant decrease zero irreversible reaction equilibrium/steady-state levels). They require intentional repetitive derive rate data use kinetics, which logically follows after presentation curves. Progress clarify topics such as steady state product inhibition. present FOB graded that allow approach analyze simple reactions (noncatalyzed first-order single substrate enzyme-catalyzed), coupled (steady state, inhibitions, signaling motifs), finally entire metabolic pathways. explains how write (or use) binding, differential, mass action equations schemes. Computational graphs performed behind scenes using imported from Vcell Biomodels. MiniSidewinder: NIH/NIGMS (Grant R01-GM123032-04) LibreText: Department Education Open Textbook Pilot Project (FIPSE), UC Davis Office Provost, Library, California State University Affordable Learning Solutions Program, Merlot.