Abstract 1974 Exploring the Link Between Early Life Adversity, Sleep Patterns, and Eating Using Automated High-Throughput Behavioral Recognition Software

• DOI: 10.1016/j.jbc.2024.106293
• Publication Date: 2024-03-25T21:54:01Z
• Authors: Allison Rhee; Timothy Simon; Julio Sierra; Giara Wright; Julius Horn; Arianna Williams; Johnny Figueroa
• Keywords: Sleep; Link (geometry)

Early social isolation (SI) stress can negatively impact sleep habits and weight management throughout life. Strong evidence from studies in both humans rodents indicates that SI impacts increases the preference for high-fat calorically dense foods. These effects are significant risk factors associated with obesity. However, whether SI-induced shifts predict eating later life has not been evaluated. Furthermore, utilizing home-cage monitoring systems software capable of unbiasedly tracking recording high-throughput behavioral data applied to explore this association. This study sought identify sleep-related biomarkers predictive using automated recognition (BRS). We hypothesized early-life would provide quantifiable associations strong value between resting patterns food consumption used an animal model where rats were housed pairs (n=12) or isolated (n=12). Experimental housing began adolescence continued into young adulthood 7 weeks. An array assessments was monitored weekly during rat's active phase/dark cycle Noldus Phenotyper/Ethovision XT BRS. In study, we evaluated behavior as a proxy sleep. Resting behaviors defined minimal activity while sitting lying down, sleep, lack interest surroundings. adulthood, provided all animals Western-like diet (WD) (43% kcal fat) evaluate preferences desirability. Results correlating adolescent frequencies adult demonstrate association these two factors. During early (week 2), paired had higher correlation (r = -0.4134, p 0.1816) than -0.1794, 0.5769). Late 7) demonstrated highest negative -0.6094, 0.0354) compared -0.001337, 0.9967). shift coefficients late supports hypothesis impacted by is indicator Interestingly, when WD daytime, -0.80, 0.002) their counterparts -0.37, 0.24). Still, effect lost given night. support disruptions be factor adulthood. summary, BRS enabled us shed light on relationship experiences, dietary choices. opened avenues future research interventions promote healthier outcomes those who experience adversities. partly supported NIH (DK124727, GM060507, MD006988) Loma Linda University School Medicine GRASP Seed Funds JDF.