High throughput assay for the determination of lumefantrine in plasma

• DOI: 10.1016/j.jchromb.2005.06.022
• Publication Date: 2005-07-12T21:55:08Z
• Authors: A. Annerberg; T. Singtoroj; P. Tipmanee; N.J. White; N.P.J. Day; N. Lindegårdh
• Keywords: Antimalarials; Fluorenes; 03 medical and health sciences; Lumefantrine; 0302 clinical medicine; Ethanolamines; Humans; Reproducibility of Results; Chromatography, Liquid; 3. Good health

A high throughput bioanalytical assay for the determination of lumefantrine in plasma has been developed and validated extensively. The within-day precisions for lumefantrine were 5.2, 3.5 and 2.5% at 200, 2000 and 15000 ng/mL, respectively. The between-day precisions were 4.0, 2.8 and 3.1% at 200, 2000 and 15000 ng/mL, respectively. The lower limits of quantification (LLOQ) and the limits of detection (LOD) were 25 and 10 ng/mL, respectively using 0.250 mL plasma. The average recovery of lumefantrine was 85% and independent upon concentration. The use of 96-well plate format and short chromatographic run has increased the daily sample throughput four times. The assay is particularly suitable for large therapeutic drug monitoring studies using day 7 sampling.