Enantioselective three-dimensional high-performance liquid chromatographic determination of amino acids in the Hayabusa2 returned samples from the asteroid Ryugu

• DOI: 10.1016/j.jcoa.2024.100134
• Publication Date: 2024-05-10T07:35:45Z
• Authors: Aogu Furusho; Chiharu Ishii; Takeyuki Akita; Mai Oyaide; Masashi Mita; Hiroshi Naraoka; Yoshinori Takano; Jason P. Dworkin; Yasuhiro Oba; Toshiki Koga; Kazuhiko Fukushima; Dan Aoki; Minako Hashiguchi; Hajime Mita; Yoshito Chikaraishi; Naohiko Ohkouchi; Nanako O. Ogawa; Saburo Sakai; Daniel P. Glavin; Jamie E. Elsila; Eric T. Parker; José C. Aponte; Hannah L. McLain; Francois-Regis Or...; Véronique Vuitton; Roland Thissen; Cédric Wolters; Philippe Schmitt-...; Alexander Ruf; Junko Isa; Norbert Hertkorn; John M. Eiler; Toshihiro Yoshimura; Haruna Sugahara; Heather V. Graham; Yoshihiro Furukawa; Daisuke Araoka; Satoru Tanaka; Takaaki Yoshikawa; Fumie Kabashima; Kazunori Sasaki; Hajime Sato; Tomoya Yamazaki; Morihiko Onose; Mayu Morita; Yuki Kimura; Kuniyuki Kano; Junken Aoki; Kosuke Fujishima; Shin-ichiro Nomura; Shogo Tachibana; Tomoki Nakamura; Takaaki Noguchi; Ryuji Okazaki; Hikaru Yabuta; Hisayoshi Yurimoto; Kanako Sakamoto; Toru Yada; Masahiro Nishimura; Aiko Nakato; Akiko Miyazaki; Kasumi Yogata; Masanao Abe; Tomohiro Usui; Makoto Yoshikawa; Takanao Saiki; Satoshi Tanaka; Fuyuto Terui; Satoru Nakazawa; Tatsuaki Okada; Sei-ichiro Watanabe; Yuichi Tsuda; Kenji Hamase
• Keywords: 0301 basic medicine; 03 medical and health sciences; QD71-142; 330; Three-dimensional HPLC; Asteroid; Amino acids; Meteorite; Enantiomer separation; Analytical chemistry; Hayabusa2

The chirality of amino acids in extraterrestrial materials may provide an insight into the origin essential l-enantiopure terrestrial biosphere. In 2020, Hayabusa2 mission succeeded bringing back surface from C-type asteroid (162173) Ryugu to Earth. Amino were one targeted organic molecules be studied samples. To analyze various structural isomers acids, which expected present, limited amount returned samples, development a highly-sensitive and selective analytical method was necessary. present study, three-dimensional high-performance liquid chromatography (3D-HPLC) system has been developed for enantioselective determination five proteinogenic three non-proteinogenic sample separated by reversed-phase, anion-exchange columns after fluorescence derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiozole. applicability samples evaluated analyzing several types carbonaceous meteorites before applying analysis all target successfully determined quantitatively. Non-proteinogenic including 2-amino-n-butyric acid, isovaline norvaline, rarely environment, found as almost racemic mixtures 47.1 55.2 %l.