Effects of environmental hypoxia on the goldfish skeletal muscle: Focus on oxidative status and mitochondrial dynamics

• DOI: 10.1016/j.jconhyd.2024.104299
• Publication Date: 2024-01-13T02:08:11Z
• Authors: Mariacristina Filice; Alessia Caferro; Alfonsina Gattuso; Emilio Sperone; Claudio Agnisola; Caterina Faggio; Maria Carmela Cerra; Sandra Imbrogno
• Keywords: Hypoxia; TBARS; TFAM

The skeletal muscle is a highly plastic tissue. Its ability to respond external stimuli and challenges allows it face the functional needs of organism. In goldfish Carassius auratus, model hypoxia resistance, exposure reduced oxygen accompanied by an improvement swimming performance, relying on sustained contractile behavior muscle. At moment, limited information available mechanisms underlying these responses. We here evaluated effects short- (4 days) long- (20 term moderate water white muscle, focusing oxidative status mitochondrial dynamics. No differences in lipid peroxidation, measured as 2-thiobarbituric acid-reacting substances (TBARS), oxidatively modified proteins (OMP) were detected animals exposed with respect their normoxic counterparts. Exposure short-term was characterized enhanced SOD activity expression, paralleled increased levels Nrf2, regulator antioxidant cell response, HSP70, chaperone also acting redox sensor. expression markers biogenesis (TFAM) abundance (VDAC) mtDNA/nDNA ratio similar under normoxia both long-term hypoxia, thus excluding rearrangement apparatus. Only increase PGC1α (a transcription factor involved dynamics) after 20 days hypoxia. Our results revealed novel aspects molecular that may sustain response contributing adequate tissue function organism requirements.