Hollow microneedle-mediated intradermal delivery of model vaccine antigen-loaded PLGA nanoparticles elicits protective T cell-mediated immunity to an intracellular bacterium

• DOI: 10.1016/j.jconrel.2017.09.017
• Publication Date: 2017-09-14T08:21:09Z
• Authors: Anne Marit de Groot; Guangsheng Du; Juha Mönkäre; Anouk C.M. Platteel; Femke Broere; Joke A. Bouwstra; Alice J.A.M. Sijts
• Keywords: PLGA; Cell mediated immunity; Cellular immunity; Intracellular parasite

The skin is an attractive organ for immunization due to the presence of a large number epidermal and dermal antigen-presenting cells. Hollow microneedles allow precise non-invasive intradermal delivery vaccines. In this study, ovalbumin (OVA)-loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles with without TLR3 agonist poly(I:C) were prepared administered intradermally by hollow microneedles. capacity PLGA induce cytotoxic T cell response, contributing protection against intracellular pathogens, was examined. We show that single injection OVA-loaded nanoparticles, compared soluble OVA, primed both adoptively transferred antigen-specific naïve transgenic CD8 + CD4 cells markedly high efficiency. Applying triple protocol, also endogenous OVA-specific Immune following in particular anionic co -encapsulated OVA poly(I:C), provided recombinant strain bacterium Listeria monocytogenes , secreting OVA. Taken together, we nanoparticle formulation excellent system protein antigen into protective cellular immune responses can be induced using immunizations.