Cationic lipids as one-component vaccine adjuvants: A promising alternative to alum

• DOI: 10.1016/j.jconrel.2018.08.020
• Publication Date: 2018-08-12T21:33:24Z
• Authors: Malvina Pizzuto; Pascal Bigey; Anne-Marie Lachagès; Céline Hoffmann; Jean-Marie Ruyssc...; Virginie Escriou; Caroline Lonez
• Keywords: [SDV.BIO]Life Sciences [q-bio]/Biotechnology; [SDV.IMM] Life Sciences [q-bio]/Immunology; Interleukin-1beta; LIPOSOMES; Immunostimulant; Inflammasome; Mice; 03 medical and health sciences; Drug Delivery Systems; 0302 clinical medicine; Adjuvants, Immunologic; Cations; Cell Line, Tumor; Polyamines; Animals; Humans; Adjuvant; Delivery system; Cationic lipids; Vaccines; IFN-GAMMA; One-component vaccine adjuvants; Vaccination; LIPOPLEXES; DNA; Sciences bio-médicales et agricoles; Lipids; Toll-Like Receptor 2; Lipopolyamines; Toll-like receptors; [SDV.BIO] Life Sciences [q-bio]/Biotechnology; 3. Good health; Toll-Like Receptor 4; DIFFERENTIATION; HEK293 Cells; RAW 264.7 Cells; INFLAMMASOME; [SDV.IMM]Life Sciences [q-bio]/Immunology; Alum Compounds; Female; DIC14-AMIDINE; REGULATOR; Vaccine; DENDRITIC CELL; Nanocarriers; GENERATION

Effective vaccine formulations consist of several components: an antigen carrier, the antigen, a stimulator of cellular immunity such as a Toll-like Receptors (TLRs) ligand, and a stimulator of humoral response such as an inflammasome activator. Here, we investigated the immunostimulatory and adjuvant properties of lipopolyamines, cationic lipids used as gene carriers. We identified new lipopolyamines able to activate both TLR2 and TLR4 and showed that lipopolyamines interact with TLRs via a mechanism different from the one used by bacterial ligands, activating a strong type-I IFN response, pro-inflammatory cytokines and IL-1β secretion. The TLR and inflammasome stimulations, together with the antigen carrier properties of lipopolyamines, resulted in both humoral and cellular immunity in mice vaccinated against OVA and make lipopolyamines promising one-component vaccine adjuvants.