Sida tuberculata: In vitro cytotoxicity and in vivo anti-inflammatory effect

• DOI: 10.1016/j.jep.2021.114956
• Publication Date: 2021-12-26T14:51:42Z
• Authors: Hemerson Silva da...; Marí Castro Santos; Marcio Tavares Costa; Andréia Salgueiro; Morgana Duarte da...; Daniele Rubert No...; Cristiano Jesse; Michel Mansur Mac...; Luís Flávio Souza...; Vanderlei Folmer; Andreas S.L. Mendez
• Keywords: Inflammation; Male; 0301 basic medicine; Carcinoma, Hepatocellular; Plant Extracts; Sida Plant; Liver Neoplasms; Anti-Inflammatory Agents; Breast Neoplasms; Hep G2 Cells; Peritonitis; Antineoplastic Agents, Phytogenic; Cell Line; Disease Models, Animal; Mice; 03 medical and health sciences; MCF-7 Cells; Animals; Humans; Female

Sida tuberculata R. E. Fries (Malvaceae) is a pioneer species considered a weed in farm fields in Southern Brazil. Widely distributed in South Brazil, S. tuberculata is popularly used to treat inflammatory conditions.The current study aimed to assess the in vitro cytotoxic and in vivo anti-inflammatory properties of S. tuberculata.Initially, extracts obtained from leaves (STLE) and roots (STRE) were submitted to cytotoxicity tests using human leukocytes (non-malignant cell line) and HepG2 and MCF-7 (tumor cell lines). In sequence, anti-inflammatory properties were investigated against carrageenan-induced peritonitis model.In vitro analyses displayed a significant decrease in human leukocytes viability without genotoxic damage. IC50 results from tumor cells presented significant decrease in cell viability, slightly more pronounced for STRE. In addition, STLE significantly inhibited the inflammatory and oxidative parameters (TBARS, NPSH, SOD, MPO activity, cell influx, and cytokines release).Our findings indicate S. tuberculata extracts have cytotoxic potential more pronounced on tumor cell lines, as well as leaves extract shows a significant reduction in acute inflammation process, as already reported for Sida genus and specifically for this species.