Mongolian medicine formulae Ruda-6 alleviates indomethacin-induced gastric ulcer by regulating gut microbiome and serum metabolomics in rats

• DOI: 10.1016/j.jep.2023.116545
• Publication Date: 2023-05-17T16:09:09Z
• Authors: Lan Feng; Terigele Bao; Laxinamujila Bai; Xiyele Mu; Na Ta; Minglan Bao; Yonghui Li; Junqing Zhang; Minghai Fu; Yongsheng Chen
• Keywords: Eubacterium; Ulcer index

Ruda-6 (RD-6), a typical traditional Mongolian medicine formulae consisting of 6 herbs, has been traditionally used in treating gastric disorders. Even though it shown to protect against ulcers (GU) animal models, the gut microbiome and serum metabololite-related mechanisms that prevent GU are not well understood.This study was conducted evaluate gastroprotective mechanism RD-6 associated with alteration metabolic profiles rats.RD-6 (0.27, 1.35 2.7 g/kg) or ranitidine (40 mg/kg) were orally administered rats for three weeks before induction ulcer using indomethacin (30 mg/kg, single oral dose). The index, area, H&E staining, levels TNF-α, iNOS, MPO MDA quantified inhibitory effects RD-6. Then, 16S rRNA gene sequencing combined LC-MS profiling performed investigate effect on microbiota metabolites rats. Moreover, spearman analysis calculate correlation coefficient between different metabolites.RD-6 inhibited lesion damage caused by rats, decreased index 50.29% (p < 0.05), reduced tissue. Additionally, reshaped diversity microbial composition, reversed bacteria including [Eubacterium]_xylanophilum group, Sellimonas, Desulfovibrio, UCG-009, increased Aquamicrobium induction. Furthermore, regulated amino acids organic acids, these affected involved taurine hypotaurine metabolism tryptophan metabolism. Spearman revealed perturbed closely related changes differential metabolites.In view results, present suggests ameliorating via modulating intestinal their metabolites.