Porcine bone collagen peptides promote osteoblast proliferation and differentiation by activating the PI3K/Akt signaling pathway

• DOI: 10.1016/j.jff.2019.103697
• Publication Date: 2019-12-02T15:45:55Z
• Authors: Lingyu Zhu; Yingying Xie; Boting Wen; Mengliang Ye; Yusi Liu; Khandaker Md Shar...; Huimei Cai; Chunhui Zhang; Fengzhong Wang; Fengjiao Xin
• Keywords: PI3K/Akt; Osteoblasts; 0404 agricultural biotechnology; Nutrition. Foods and food supply; Collagen peptides; Apoptosis; TX341-641; 04 agricultural and veterinary sciences; Cell cycle; 3. Good health

Collagen peptides are widely used in food and medicine industries. Given the significant role of collagen bone structure, considered promising for osteoporosis prevention treatment. To elucidate their effect on osteoporosis, we extracted porcine collagen, digested it into peptides, investigated biological function using osteoblast MC3T3-E1 cells. The structure was relatively stable prepared with different molecular weights (MWs) via hydrolysis two commercially-available enzymes (alcalase/trypsin). Low-MW (<1 kDa) mainly comprised tripeptides, increased proliferation promoted cell cycle progression by upregulating CDK-2, CDK-4, Cyclin B1, D1, inhibited apoptosis decreasing Bax/Bcl-2 cleaved caspase-3 expression. Meanwhile, they upregulated p-Akt downregulated PTEN, indicating that low-MW differentiation activating PI3K/Akt pathway. Thus, report mechanism through which bone-derived could be to prevent treat osteoporosis.