β-Glucan from Lyophyllum decastes regulates gut microbiota and plasma metabolites in high-fat diet-induced obese mice

• DOI: 10.1016/j.jff.2024.106166
• Publication Date: 2024-04-10T11:13:11Z
• Authors: Bin Sun; Zhenzhen Huang; Ying Xiao; Fangfang Zhang; Liang Pan; Ling Yu; Lin Ye; Yanfang Liu; Xiaodan Liu; Qi Xu; Jieying Liu; Shiyue Yan
• Keywords: Prebiotic; Occludin

This study aimed to investigate the effects of Lyophyllum decastes β-glucan (LDBG) in preventing high-fat diet (HFD)-induced obesity male C57BL/6 mice, focusing on fecal gut microbiota (GM) and plasma metabolomics. LDBG treatment led a reduction body weight an improvement dyslipidemia HFD-fed mice. Additionally, decreased pro-inflammatory factors (TNF-α, IL-1β, IL-6) liver abdominal adipose tissue lowered lipopolysaccharide leptin levels plasma. also up-regulated mRNA intestinal epithelial tight junction proteins, such as Occludin, Claudin-1, zonula occludens-1 colon tissue. Furthermore, 16S rRNA gene sequencing data suggested that reduced relative abundance Faecalibaculum Ileibacterium while increasing Bifidobacterium Oscillospiraceae. Untargeted metabolomics showed significantly promoted Tryptophan metabolism, Glycerophospholipid Citrate cycle, closely correlating with GM changes, particularly Oscillospiraceae genera. demonstrates potential prebiotic prevention by regulating metabolite pathways.