Phylogenomic analysis of CTX-M-15–producing Enterobacter hormaechei belonging to the high-risk ST78 from animal infection: another successful One Health clone?

• DOI: 10.1016/j.jgar.2022.02.010
• Publication Date: 2022-02-18T17:08:45Z
• Authors: Brenda Cardoso; Fábio P. Sellera; Elder Sano; Fernanda Esposito; Lourdes A.V. Seabra; Milton R. Azedo; Fabio C. Pogliani; Nilton Lincopan
• Keywords: Indel; Enterobacteriaceae Infections

Extended-spectrum β-lactamase (ESBL)–producing Enterobacter cloacae complex (ECC) members have been a leading cause of severe infections in hospital setting and lately recognized as important pathogens for animals. In this article, we report phylogenomic data multidrug-resistant CTX-M-15–positive E. hormaechei belonging to ST78 isolated from calf with omphalitis. Genomic DNA was extracted sequenced using the Illumina NextSeq platform. De novo assembly performed by Unicycler silico prediction accomplished curated bioinformatics tools. Single nucleotide polymorphism (SNP)-based comparative analysis conducted publicly available ECC genomes ST78. The genome size calculated at 3 8465 40 bp, comprising 4717 total genes, rRNAs, 43 tRNAs, 7 ncRNAs, 74 pseudogenes. animal-associated (ECBEZ strain) harboured blaCTX-M-15 ESBL gene addition other critically resistance genes conferring β-lactams, aminoglycosides, fosfomycin, phenicol, quinolones, sulphonamides, tetracyclines, trimethoprim. Phylogenetic revealed that ECBEZ is closely related human-isolated strains Asian African countries. Phylogenomic CTX-M-15-producing animal infection reveals successful One Health clone among members. Furthermore, presented study reinforce urgent need monitor ESBL-producing veterinary settings.