Korean Red Ginseng extract reduces hypoxia-induced epithelial-mesenchymal transition by repressing NF-κB and ERK1/2 pathways in colon cancer

• DOI: 10.1016/j.jgr.2017.03.008
• Publication Date: 2017-03-29T04:23:37Z
• Authors: Eui Joo Kim; Kwang An Kwon; Young Eun Lee; Ju Hyun Kim; Se-Hee Kim; Jung Ho Kim
• Keywords: Hypoxia

The incidence of colorectal cancer (CRC) is increasing, with metastasis newly diagnosed CRC reported in a large proportion patients. However, the effect Korean Red Ginseng extracts (KRGE) on epithelial to mesenchymal transition (EMT) unknown. Therefore, we examined mechanisms by which KRGE regulates EMT hypoxic conditions. Human cell lines HT29 and HCT116 were incubated under (1% oxygen) normoxic (21% Western blot analysis real-time PCR used evaluate expression markers presence KRGE. Furthermore, performed scratched wound healing, transwell migration, invasion assays monitor whether affects migratory invasive abilities cells KRGE-treated displayed attenuated vascular endothelial growth factor (VEGF) mRNA levels hypoxia-inducible factor-1α (HIF-1α) protein repressed Snail, Slug, Twist integrin αVβ6 levels. hypoxia-repressed E-cadherin was restored cells; blocked migration colon repressing NF-κB ERK1/2 pathways hypoxia. inhibits hypoxia-induced cells.