Ginseng-derived nanoparticles induce skin cell proliferation and promote wound healing

• DOI: 10.1016/j.jgr.2022.07.005
• Publication Date: 2022-08-17T09:46:23Z
• Authors: Song Yang; Shuyan Lu; Limei Ren; Shuai Bian; Daqing Zhao; Meichen Liu; Jiawen Wang
• Keywords: HaCaT

Past studies suggested that ginseng extracts and ginseng-derived molecules exerted significant regulatory effects on skin. However, no reports have described the of nanoparticles (GDNPs) skin cell proliferation wound healing. In this study, we investigated whether GDNPs regulate cells promote healing in a mouse model.GDNPs were separated purified via differential centrifugation sucrose/D2O gradient ultracentrifugation. GDNP uptake, cycle progression measured by confocal microscopy, CCK-8 assay flow cytometry, respectively. Cell migration angiogenic assessed scratch tube formation assay, ELISA was used to detect extracellular matrix secretion. The relevant signaling pathway confirmed western blotting. observation, HE staining, blotting.GDNPs possessed essential features exosomes, they accumulated cells. Treatment with notably enhanced HaCaT, BJ HUVECs. also HaCaT HUVECs angiogenesis increased secretion MMP-1, fibronectin-1, elastin-1, COL1A1 all three lines. regulated through ERK AKT/ mTOR pathways. Furthermore, facilitated decreased inflammation can AKT/mTOR thus represent an alternative treatment for chronic wounds.