Identification and characterization of a plasmid co-harboring blaCTX-M-55 and blaTEM-141 in Escherichia albertii from broiler in China1

• DOI: 10.1016/j.jia.2023.12.038
• Publication Date: 2024-01-03T16:00:45Z
• Authors: Weiqi Guo; Di Wang; Xinyu Wang; Zhiyang Wang; Hong Zhu; Jiangang Hu; Beibei Zhang; Jingjing Qi; Mingxing Tian; Yanqing Bao; Na Li; Wanjiang Zhang; Shao-hui Wang
• Keywords: Multilocus sequence typing

The inappropriate use of cephalosporins lead to the occurrence and global spread bacteria resistant these antimicrobials. In this study, we isolated four Escherichia albertii (E. albertii) strains from broilers in Eastern China. antimicrobial susceptibility genomic characterization E. isolates were determined. Our results revealed that exhibited resistance tetracyclines, chloramphenicol, β-lactams, aminoglycosides, polymyxin B, sulfonamides, quinolones, other Among them, EA04 isolate was multidrug harbored extended-spectrum β-lactamases (ESBL) genes blaCTX-M blaTEM. Whole genome sequencing core-genome Multilocus sequence typing (cgMLST) based on all ST4638 for inferred highly probable epidemiological links between selected human isolates. Additionally, ESBL blaTEM-141 blaCTX-M-55 coexistent an approximately 75 kb IncFII plasmid pEA04.2 EA04. Comparative analysis indicated located IS15-blaCTX-M-55-wbuC-blaTEM-141-IS26 region, which similar structures identified various bacteria. Furthermore, could be transferable coli EC600 third-generation cephalosporins. These suggested chicken potentially serve as a reservoir albertii, increases risk horizontal transfer humans, animals environment.