SOX2 Epidermal Overexpression Promotes Cutaneous Wound Healing via Activation of EGFR/MEK/ERK Signaling Mediated by EGFR Ligands

• DOI: 10.1016/j.jid.2019.02.004
• Publication Date: 2019-02-14T16:55:41Z
• Authors: Akihiko Uchiyama; Subhashree Nayak; Rose Graf; Michael Cross; Kowser Hasneen; J. Silvio Gutkind; Stephen R. Brooks; Maria I. Morasso
• Keywords: Keratinocytes; Male; 0301 basic medicine; MAP Kinase Signaling System; Cells; Primary Cell Culture; Ligands; Mice; 03 medical and health sciences; Models; Animals; RNA-Seq; Cells, Cultured; Cell Proliferation; Skin; Wound Healing; Cultured; Animal; SOXB1 Transcription Factors; Up-Regulation; 3. Good health; ErbB Receptors; Models, Animal; Female; Heparin-binding EGF-like Growth Factor; Signal Transduction

Oral mucosa contains a unique transcriptional network that primes oral wounds for rapid resolution in humans. Our previous work identified genes that were consistently upregulated in the oral mucosa and demonstrated that induction of one of the identified genes, transcription factor SOX2, promoted cutaneous wound healing in mice. In this study, we investigated the molecular and cellular mechanisms by which SOX2 accelerates wound healing in skin. RNA-sequencing analysis showed that SOX2 induced a proliferative and wound-activated phenotype in skin keratinocytes prior to wounding. During wound healing, SOX2 induced proliferation of epithelial and connective tissue cells and promoted angiogenesis. Chromatin immunoprecipitation assay revealed that SOX2 directly regulates expression of EGFR ligands, resulting in activation of EGFR. In vitro, skin keratinocytes overexpressing SOX2 promoted cell migration via the EGFR/MEK/ERK pathway. We conclude that induction of SOX2 in skin keratinocytes accelerates cutaneous wound healing by promoting keratinocyte migration and proliferation, and enhancement of angiogenesis via upregulation of EGFR ligands and activation of EGFR/MEK/ERK pathway. Through the identification of putative cutaneous SOX2 targets, such as HBEGF, this study opens venues to determine clinical targets for treatment of skin wounds.