Cell-derived microvesicles in infective endocarditis: Role in diagnosis and potential for risk stratification at hospital admission

• DOI: 10.1016/j.jinf.2019.06.005
• Publication Date: 2019-06-14T15:10:30Z
• Authors: Milton Henriques ...; Teresa Cristina A...; Andréa Teixeira-C...; Marcela de Lima M...; Lorena Júnia de S...; Matheus Fernandes...; Rodrigo Matos Pin...; Pedro Henrique Ol...; Tijmen Hermen Ris; Jonathas Teixeira...; Lívia Silva Araúj...; Maria Carmo Perei...
• Keywords: Adult; Male; Endocarditis; Middle Aged; Prognosis; Risk Assessment; 3. Good health; Hospitalization; 03 medical and health sciences; 0302 clinical medicine; Cell-Derived Microparticles; Humans; Female; Disease Susceptibility; Hospital Mortality; Symptom Assessment; Biomarkers; Aged

To characterize the plasmatic profile of cell-derived microvesicles (MVs) at diagnosis and during the treatment of patients with infective endocarditis (IE).Blood samples from 57 patients with IE were obtained on 3 consecutive moments: upon admission (T0), at 2 weeks (T1), and at the end of treatment (T2), and were compared with 22 patients with other bacterial infections. MPs were measured by flow cytometry and labeled for specific cell markers of CD45 (leukocytes), CD66b (neutrophils), CD14 (monocytes), CD41a (platelets), CD51 (endothelial cells), CD3 (T lymphocyte) and CD235a (erythrocytes).MVs from platelets (pltMVs), leukocytes (leukMVs), neutrophils (neutMVs), monocytes (monoMVs) and lymphocytes (lymphMVs) were significantly more elevated in the patients with IE, compared to the patients with other bacterial infections, despite comparable age, sex, blood counts and C-reactive protein levels. MVs values revealed a relatively stable pattern over time in IE, except for a significant increase in leukMVs and neutMVs in T1. LeukMVs (p = 0.011), neutMVs (p = 0.010), monoMVs (p = 0.016) and lymphMVs (p = 0.020), measured at admission, were significantly higher in IE patients that died during hospitalization in comparison with those that survived. In a multivariable analyses, the levels of neutMVs remained as an independent factor associated with mortality (odds ratio 2.203; 95% confidence interval 1.217 - 3.988; p = 0.009), adjustment for heart failure during the treatment.Plasma levels of pltMVs, leukMVs, neutMVs, monoMVs and lymphMVs were significantly more elevated in patients with IE than in patients with other bacterial infections at hospital admission. Furthermore, neutMVs at admission have been identified as an independent predictor of mortality in patients with IE. Thus, cell derived MPs may become an important tool in the differential diagnosis and mortality risk assessment early in the course of IE suspected cases.