Smoking induces different expression of miR-320b and miR-10b-5p in plasma extracellular vesicles of non-small cell lung cancer patients

• DOI: 10.1016/j.jlb.2025.100291
• Publication Date: 2025-03-09T06:39:03Z
• Authors: Markus Yovian Wid...; Septelia Inawati ...; Achmad Mulawarman...; Donny Lukmanto; Yuniar Harris Pra...; Noorwati Sutandyo
• Keywords: Extracellular Vesicles; Extracellular vesicles

Previous studies found that cigarette smoke (CS) exposure could induce NSCLC malignancy and miRNA dysregulation. Yet, the association of CS-induced dysregulation has not been clearly understood. This study aimed to evaluate effect CS in smokers on expression miR-10b-5p miR-320b extracellular vesicles (EVs) from patients. Bioinformatic analysis was conducted validate candidates. Blood tissue samples were collected patients (n = 21) with smoking non-smoking history. EVs isolated plasma miRNAs extracted EVs. The relative analyzed compared. In silico identified as potential biomarkers for diagnosing smokers. Experimental revealed differential EVs-associated histories. significantly overexpressed smoker (p 0.000), while lower this group 0.018). Additionally, intensity influenced expression, higher correlating increased decreased expression. ROC demonstrated a superior source compared diagnostics. showed diagnostic performance (AUC 0.878; 0.739) 0.628; 0.559). induces different EV-related be utilized prognostic biomarker