High formability Mg-Zn-Gd wire facilitates ACL reconstruction via its swift degradation to accelerate intra-tunnel endochondral ossification

• DOI: 10.1016/j.jma.2022.12.006
• Publication Date: 2022-12-25T03:01:38Z
• Authors: Xuan He; Ye Li; Hongwei Miao; Jiankun Xu; Michael Tim-yun Ong; Chenmin Wang; Lizhen Zheng; Jiali Wang; Le Huang; Haiyue Zu; Zhi Yao; Jie Mi; Bingyang Dai; Xu Li; Patrick Shu-hang ...; Guangyin Yuan; Ling Qin
• Keywords: Fibrocartilage; Acetabular Labrum; Endochondral ossification

After reconstructing the anterior cruciate ligament (ACL), unsatisfactory bone tendon interface healing may often induce tunnel enlargement at early stage. With good biological features and high formability, Magnesium-Zinc-Gadolinium (ZG21) wires are developed to bunch graft for matching during transplantation. Microstructure, tensile strength, degradation, cytotoxicity of ZG21 wire evaluated. The rabbit model is used assessing effects by Micro-CT, histology, mechanical test. SEM/EDS, immunochemistry, in vitro assessments performed investigate underlying mechanism. Material tests demonstrate formability as surgical suture. Micro-CT shows degradation accelerates formation, histologically with earlier more fibrocartilage regeneration interface. test higher ultimate load group. SEM/EDS presents triggered calcium phosphate (Ca-P) deposition. IHC results upregulation Wnt3a, BMP2, VEGF phase TGFβ3 Type II collagen late healing. In also confirmed Ca-P metal extract could elevate expression β catenin, ocn opn stimulate osteogenesis. Ex vivo clinical samples indicated suturing did not weaken loading human tissue. conclusion, feasible bunching. Its products accelerated intra-tunnel endochondral ossification stage therefore enhanced bone-tendon ACL reconstruction.