Biogenesis of a Bacteriophage Long Non-Contractile Tail

• DOI: 10.1016/j.jmb.2021.167112
• Publication Date: 2021-06-18T07:08:16Z
• Authors: Anait Seul; Sandrine Brasilès; Isabelle Petitpas; Rudi Lurz; Valérie Campanacci; Christian Cambillau; Frank Weise; Mohamed Zairi; Paulo Tavares; Isabelle Auzat
• Keywords: [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology; 0301 basic medicine; 0303 health sciences; [SDV.BIBS] Life Sciences [q-bio]/Quantitative Methods [q-bio.QM]; Virus Assembly; Virion; Genome, Viral; Viral Tail Proteins; Siphoviridae; [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology; 03 medical and health sciences; Capsid; [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM]; Bacillus subtilis; Molecular Chaperones

Siphoviruses are main killers of bacteria. They use a long non-contractile tail to recognize the host cell and to deliver the genome from the viral capsid to the bacterial cytoplasm. Here, we define the molecular organization of the Bacillus subtilis bacteriophage SPP1 ~ 6.8 MDa tail and uncover its biogenesis mechanisms. A complex between gp21 and the tail distal protein (Dit) gp19.1 is assembled first to build the tail cap (gp19.1-gp21Nter) connected by a flexible hinge to the tail fiber (gp21Cter). The tip of the gp21Cter fiber is loosely associated to gp22. The cap provides a platform where tail tube proteins (TTPs) initiate polymerization around the tape measure protein gp18 (TMP), a reaction dependent on the non-structural tail assembly chaperones gp17.5 and gp17.5* (TACs). Gp17.5 is essential for stability of gp18 in the cell. Helical polymerization stops at a precise tube length followed by binding of proteins gp16.1 (TCP) and gp17 (THJP) to build the tail interface for attachment to the capsid portal system. This finding uncovers the function of the extensively conserved gp16.1-homologs in assembly of long tails. All SPP1 tail components, apart from gp22, share homology to conserved proteins whose coding genes' synteny is broadly maintained in siphoviruses. They conceivably represent the minimal essential protein set necessary to build functional long tails. Proteins homologous to SPP1 tail building blocks feature a variety of add-on modules that diversify extensively the tail core structure, expanding its capability to bind host cells and to deliver the viral genome to the bacterial cytoplasm.