Mussel-inspired extracellular matrix-mimicking hydrogel scaffold with high cell affinity and immunomodulation ability for growth factor-free cartilage regeneration

• DOI: 10.1016/j.jot.2022.02.006
• Publication Date: 2022-03-10T04:13:46Z
• Authors: Donglin Gan; Yanan Jiang; Yuelin Hu; Xiao Wang; Qiguang Wang; Kefeng Wang; Chaoming Xie; Lu Han; Xiong Lu
• Keywords: Chondrogenesis

Injury to articular cartilage cause certain degree of disability due poor self-repair ability tissue. To promote regeneration, it is essential develop a scaffold that properly mimics the native extracellular matrix (ECM) in aspect compositions and functions.A mussel-inspired strategy was developed construct an ECM-mimicking hydrogel by incorporating polydopamine-modified hyaluronic acid (PDA/HA) complex into dual-crosslinked collagen (Col) for growth factor-free regeneration. The adhesion, proliferation, chondrogenic differentiation cells on were examined. A well-established full-thickness defect model knee rabbits used evaluated efficacy functionality engineered Col/PDA/HA scaffold.The PDA/HA incorporated-hydrogel with catechol moieties exhibited better cell affinity than bare negatively-charged HA incorporated scaffold. In addition, endowed immunomodulation ability, which suppressed expression inflammatory cytokines effectively activated polarization macrophages toward M2 phenotypes. vivo results revealed showed strong inducing regeneration.The complex-incorporated overcame repellency polysaccharide-based scaffolds, facilitated adhesion clustering scaffold, therefore enhanced cell-HA interactions efficient differentiation. Moreover, modulated immune microenvironment, created regenerative microenvironment enhance regeneration.This study gives insight approach tissue-inducing growth-factor-free manner, show great advantage clinical treatment. composed as major component, providing environment, promising repair.