Exosomes from CD133+ human urine-derived stem cells combined adhesive hydrogel facilitate rotator cuff healing by mediating bone marrow mesenchymal stem cells

• DOI: 10.1016/j.jot.2023.02.002
• Publication Date: 2023-02-25T04:36:32Z
• Authors: Xiaopeng Tong; Yan Xu; Tao Zhang; Chao Deng; Jinrui Xun; Deyi Sun; Daqi Xu
• Keywords: Hydrogel; 03 medical and health sciences; 0302 clinical medicine; Rotator cuff healing; RC925-935; Chondrogenic differentiation; Original Article; Bone-tendon interface; Diseases of the musculoskeletal system; Bone marrow mesenchymal stem cell; CD133+ urine-derived stem cell exosome

The inadequate regeneration of natural tissue (mainly fibrocartilage) between tendon and bone during rotator cuff (RC) repair results in an unsatisfactory quality RC healing. Cell-free therapy based on stem cell exosomes is a safer more promising approach for regeneration. Here, we investigated the effect from human urine-derived cells (USCs) their subpopulations (CD133+USCs) healing.USCs were isolated urine sorted by flow cytometry to obtain CD133+ (CD133+ USCs). Urine-derived (USC-Exos) USC-Exos) subsequently supernatant identified transmission electron microscopy (TEM), particle size analysis, Western blot. We performed vitro functional assays evaluate effects USC-Exos marrow mesenchymal (BMSCs) proliferation, migration, osteogenic differentiation, chondrogenic differentiation. In vivo experiments local injection exosome-hydrogel complexes treatment injury. healing assessed imaging, histological, biomechanical tests.CD133+ USCs positive CD29, CD44, CD73, CD90, CD133, but negative CD34 CD45. Differentiation ability test showed that both had potential osteogenic, chondrogenic, adipogenic stronger differentiation ability. could be efficiently taken up BMSCs promote However, than USC-Exos. Compared with USC-Exos, bone-tendon interface (BTI) effectively, which might related its into chondroblasts. Although two exhibited same promoting subchondral BTI, group higher histological scores properties.CD133+ hydrogel complex may become therapeutic exosomes.This first study assess specific role activation towards Further, our provides reference possible future BTI applying complex.